Moth holes: parallel lines in the tissue or separation of tissue into fine lines.

Moth holes:  parallel lines in the tissue or separation of tissue into fine lines

Causes and remedies

  • Over-agressive trimming- too fast, too thick, or both
    Slow down trimming speed, and reduce trimming micron thickness
  • Block too cold
    Do not let sit on ice too long; lightly warm the block with your finger
  • Poor tissue processing
    Soak block on ice bath to soften overprocessed, hard tissue
Microscopic view of pink and purple stained tissue sample showing various cell structures and white spaces.

Moth holes:  parallel lines in the tissue or separation of tissue into fine lines Causes and remedies