Webinar Quiz: Basic Scientific Principles of Molecular Pathology Techniques -Sakura Finetek USA

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Which of the following statements is true?(Required)

Of the various macromolecules, DNA carries out the majority of functions within a cell, including most enzymatic, structural, and signaling functions.

In human cells, RNA is the primary permanent storage molecule for genetic information.

Various types of RNA exist, including one type used to transfer genetic information from the nucleus to the cytoplasm.

Proteins are composed of long strings of nucleic acids.

Which of the following is the key functional feature of the standard polymerase chain reaction (PCR) technique?(Required)

Visualization of reaction products within the context of tissue histology.

Target specificity and amplification.

Amplification of both nucleic acid and protein targets.

Detection of large chromosomal structural abnormalities.

Which of the following statements is incorrect?(Required)

Reaction products for a real-time PCR reaction are analyzed after all cycles of the reaction have been completed.

The initial target of a reverse-transcription PCR (RT-PCR) reaction is an RNA molecule.

The three repetitive steps of a PCR reaction are denaturation, primer annealing (hybridization), and polymerization.

Denaturation is the highest-temperature step of a PCR reaction.

Which of the following is an advantage of cytogenetics over in situ hybridization (ISH) for the evaluation of chromosomal abnormalities?(Required)

Ability to perform on nonviable (fixed) tissue.

Ability to detect specific gene amplifications.

Ability to visualize within the context of tissue histology.

Ability to detect unexpected chromosomal abnormalities.

Which of the following comparisons between allele-specific PCR and chain-termination sequencing (Sanger sequencing) is incorrect?(Required)

Both of these techniques can be used to detect gene mutations.

Sanger sequencing is more sensitive than allele-specific PCR for mutation detection.

Sanger sequencing utilizes a single reaction cycle, while allele-specific PCR utilizes multiple repetitive cycles for the purpose of target amplification.

Sanger sequencing requires pre-existing knowledge of a specific expected mutation, while allele-specific PCR is useful for detection of unexpected mutations.

Webinar Quiz: Basic Scientific Principles of Molecular Pathology Techniques

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