Webinar Quiz: Cryotomy Techniques -Sakura Finetek USA

Name

This field is for validation purposes and should be left unchanged.

When the specimen has been frozen just with liquid nitrogen, what must be done first before specimen is attached to the chuck?(Required)

Use superglue to attach tissue to the chuck.

Use double-sided tape to attach tissue to the chuck.

Allow specimen to come up to cryostat temperature.

Allow tissue to thaw.

The specimen/chuck holder arm should be in what position when starting to microtome?(Required)

The arm holding the chuck is positioned as far back toward the microtome as possible.

The arm holding the chuck is positioned as far out as it can go.

The arm holding the chuck is positioned down all the way, then the knife is moved close to microtome.

The arm holding the chuck can be in any position\; just adjust the knife position.

Why is embedding liver, gastric biopsies, and lymph node all in one block for cryosectioning not a good idea?(Required)

The different specimen cutting consistencies will pull and tug on the embedding material and create folded and torn sections.

Lymph node should never be frozen sectioned since it will not stay together.

Fixative types vary for the different tissues.

It is not easy to get every tissue embedded on the same level.

What is the best possible way to embed a large piece of tissue?(Required)

Use liquid nitrogen only, without embedding material.

Use freeze spray to freeze embedding material around tissue.

Cut tissue into several smaller pieces and embed several blocks.

Press it down flat to expand the surface area.

What is the best way to store frozen sections for future use as controls or for IHC?(Required)

In the cryostat.

In the refrigerator, sealed in a box with Drierite®.

In a -20°C to -80°C freezer, sealed in a box with Drierite®.

At room temperature, sealed in a box with Drierite®.

Webinar Quiz: Cryotomy Techniques