Tissue-Tek® Cryo3® Flex Cryostat, Mohs

Innovative technology

Sakura Finetek collaborated with Mohs technicians across the United States to design a cryostat for laboratories cutting skin specimens using Mohs surgery technique.  This unique cryostat was designed to decrease the time to get complete sections, and in parallel to preserve valuable tissue.

The Tissue-Tek® Cryo3® Flex Cryostat, Mohs  produces superior sections from 1 to 99 µm using a temperature-controlled blade holder and a Winged Chuck to accurately align the block face to the blade, reducing operator's trimming time and preserving specimens.  The sturdy chuck retains its position when locked and unlocked, eliminating any time and effort spent realigning the cutting angle. Users can now leverage the newly developed Anti-Roll Rake that was designed to best mimic the preferred and widely accepted "Brush Technique" to prevent sections from rolling, which in addition, reduces the learning curve of new users making them proficient faster.

For the first time in the history of cryotomy, Sakura Finetek successfully integrated its advanced remote diagnostics technology platform, Tissue-Tek®  iSupportTM, into the hardware and software of the Tissue-Tek® Cryo3® Flex Cryostat product line. The plug-and-play connection opens communication through a secure remote service to Sakura Finetek professional technical support team, minimizing response time through fast reaction to instrument alerts, and maximizing user uptime.

A color touch LCD screen provides a visual display of critical sectioning information such as current Chamber Temperature, Section Thickness, and Trimming Thickness.  The Cryobar® offers 12 positions, allowing operators to use the Cryomold® method or direct method at the same time.  The Cryo+™ area of the Cryobar freezes to -50 C, within 5 minutes after activation.

The motorized advance and retract keys allows smooth, precise orientation of the Winged Chuck to the Blade Holder. The Blade Holder accommodates Accu-Edge® high and low-profile disposable blades.



Winged Chuck
  • Obtain complete sections faster
  • Retains position when locked and unlocked
  • Chuck wings provide technicians complete control of specimen alignment, ensuring the entire margin is available for diagnosis
1" Travel Distance
  • Cut thicker specimens
Utility Tray with Storage Space
  • Comfortably store up to 32 specimen holders in the cryo chamber keeping many blocks frozen and available to fulfill requests for immediate recuts, at any time
2 Optional Ergonomic Handles
  • Section more comfortably
  • Help prevent Repetitive Motion Disorder (RMD)
Tissue-Tek®  iSupportTM: Online Report Diagnostics Technology
  • Maximze instrument uptime
  • Get instrument support faster
Anti-Roll Rake
  • Prevent sections from rolling
Application Cryotomy
Model (#6209) Tissue-Tek® Cryo3® Flex Cryostat, Mohs
Dimensions (WxDxH) 25.8 x 29.7 x 47.0 inches
(65.4 x 75.4  x 119.4 cm) 
Weight 320 lbs (145.1 kg)
Power requirement 115/120 VAC, 50/60 Hz, 11 Amps
The instrument must be connected to an electrical outlet which supplies the proper voltage (within ± 10%)
Data interface USB and LAN
Chamber temperature
set point range
-35°C to 0°C; Chamber Temperature is maintained within ±2°C
Cryo+ temperature When activated Cryo+™ is 20°C lower than the Chamber Temperature, within 5 minutes. Lowest Cryo+™ temperature is -50°C
Section thickness settings 1 to 99 μm in 1 μm steps
Maximum specimen thickness 25 mm
Trimming range 1 to 100 μm in 1 μm steps
Specimen holder sizes Round 26 mm, 36 mm, 55 mm
(all including an alignment mark on the shaft)
Specimen orientation ±8° (XY axis), 360° (Z axis)
Chuck Winged Chuck secures the Specimen Holder to the Microtome. Wings provide techs complete control of specimen alignment, ensuring the entire margin is available for diagnosis.
Travel range Vertical: 2.5 inches (63.0 mm)
Horizontal: 1.0 inch (25.4 mm)
Defrost cycle Manual start: Defrosts the Chamber within 30 minutes, when activated
Automatic start: Defrosts the Chamber within 1 hour; start time can be set within a 24 hour period
Certifications IEC 61010-1:2010 Ed:3, IEC 61010-2-010 Ed:3, IEC 61010-2-101 Ed:2, UL 61010-1 Ed:3, CAN/CSA C22.2 # 61010-1:2012 Ed:3, EMC: CENELEC IEC/EN 61326-1, EMC: CANELEC IEC/EN 61326-2-6

Product code

Product name and quantity

4583 Tissue-Tek® O.C.T. Compound; 12/cs
4685 Accu-Edge® High Profile Microtome Blades; 500/cs
4689 Accu-Edge® Low Profile Microtome Blades; 500/cs
4728 Cryomold® Standard; 23 mm; 1200/cs
4730 Cryomold® Biopsy; 15 mm; 1200/cs
6033 Uninterrupted Power Supply (UPS), 30 minutes 
9000 VisionTek® Digital Microscope

MWEB0148 Rev.A