Webinar Quiz: Troubleshooting IHC -Sakura Finetek USA

Comments

This field is for validation purposes and should be left unchanged.

What steps is not critical to good immunohistochemistry?(Required)

Tissue processing

Fixation

Good microtomy

Drying the slides at 80° C for one hour

HIER consists of all except for:(Required)

Pre-treating tissues in citrate buffer to expose masked antigens in tissue

Pre-treating tissues in EDTA buffer to expose masked antigens in tissue

Pre-treating tissues in Tris buffer to expose masked antigens in tissue

Staining in hematoxylin

Validation of antibodies:(Required)

Is a waste of money, time and resources

Serves no value

Ensures that antibodies are not performing appropriately

Ensures the systems are performing appropriately

Ways to avoid contamination when performing IHC using automation include:(Required)

Never switch lids on antibodies or reagents

Properly label all reagent vials and containers

Never allow a pipet or tip to touch the counter top

Wear gloves when transferring or filling antibody vials

All of the above

Name some artifacts that can occur when staining IHC.(Required)

Debris from the oxidation of hematoxylin

DAB under the tissue from poor adherence

Uneven staining

Poor tissue adherence

All of the above

Only the pathologist needs to know the staining pattern of the antibodies.(Required)

True

False

The IHC tech needs to know the staining pattern of the antibodies to alert the pathologist when there is a problem.(Required)

True

False

Webinar Quiz: Troubleshooting IHC