Tissue-Tek Genie®

Clone: EP170
Host clonality: rabbit monoclonal
Control tissue: tonsil, lymph node, a subset of B-cell neoplasms
Staining pattern: membraneous and cytoplasmic
Regulatory status: IVD, FDA Class I
Product code: 8229-C010: RTU, 10 capsules; 1 pack
8229-M250: RTU, 250 tests, 1 cartridge; 1 unit


In normal, healthy serum IgA is generally the second most abundant of the Ig heavy chain classes. Igs are produced in mature B-cells and plasma cells, and the amount and type of Ig heavy chain classes can vary in different stages of B-cell maturation: Igs in the earlier stages of maturation are present in the cytoplasm, whereas Igs on the membrane surface are more characteristic of mature B-cells in the mantle zone. Benign populations of B-cells show a mix of light chain classes with varying antigen specificities, while B-cell derived neoplasms, such as B-cell lymphoma, and plasma cell neoplasms, often produce only one type of clonal Ig light chain.
Tissue-Tek Genie<sup>&reg;</sup><br /> anti-IgA An abundance of plasma cells in the gastric mucosa show a strong membrane staining reaction for IgA.

In this tonsil there is a moderate to strong cytoplasmic staining reaction in a subset plasma cells; a moderate to strong cytoplasmic and membrane staining of immunoblasts in the germinal center; some background staining in blood vessels, connective tissue and epithelial cells is due to presence of extracellular IgA.

The pancreatic acinar and islet cells show no staining reaction for IgA. Scattered plasma cells may show strong cytoplasmic reactivity. Endothelial cells and vessel lumens may also show weak staining owing to extracellular secretion of IgA proteins.
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