Thick and thin sections/How do I correct thick and thin sections?

Thick and thin: Part of the section is thick, and part of the section is thin, reflecting inconsistent staining.



Locking mechanisms loose on microtome

  • Verify locking mechanisms are tight

Blade tension too tight

  • Loosen tension on the blade

Dull blade

  • Move blade to an unused area or insert a new blade

Sectioning too quickly

  • Slow down sectioning speed

Blade angle too low

  • Increase blade angle at 1-degree increments
Back to top