Overexpansion/What causes tissue sections to ‘blow up’?

Overexpansion: cells distorted from too much water


Causes

Remedies

Water bath too hot

  • Lower temperature of water bath

Section left too long in water bath

  • Pick up sections in a timelier manner

Poor fixation

  • Ensure specimens are fixed adequately

Underprocessing

  • Reprocess appropriately

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